Fig. 5

Liver I/R injury-induced hepatocellular apoptosis was inhibited by GBDP pretreatment in mice. a Apoptotic cells were detected by TUNEL staining after 24 h of reperfusion. Bars = 100 μm. b The protein expression of apoptosis-related markers (Bax, Bcl-2, PARP-1, and caspase-3) in reperfused liver tissue was analyzed by western blotting. The lysate sample of each group was a mixture of 6 mice from the same group (n = 6 per group). β-actin was used as a loading control. The mixture sample of each group was prepared 3 times and the experiments were repeated at least 3 times. c The quantification results of Bax, d Bcl-2, e PARP-1, and f caspase-3 were shown as below. *p<0.05, **p<0.01, and ***p<0.001 vs Sham group;^#p<0.05, ^##p<0.01, and ^###p<0.001 vs I/R group