Fig. 5

13-MB induces autophagy in HUVECs. a HUVECs were treated with 13-MB in a dose-dependent manner (0.1, 1, 10 µM). The protein expression levels of LC3-II/LC3-I and SQSTM1/P62 were measured by Western blotting. b HUVECs were treated with 1 µM 13-MB, 100 nM BAF, and pretreated with BAF for 1 h followed by 13-MB. The expression levels of LC3-II/LC3-I were measured by Western blotting. c 13-MB induces autophagy in HUVECs through autophagosome quantification with microscopic analysis. The quenching/degradation expressing of both GFP and RFP in HUVECs were examined by the use of a confocal laser scanning microscope. Scale bar = 10 µm