Fig. 3
From: Ethanol extract of Ophiorrhiza pumila suppresses liver cancer cell proliferation and migration
OPE induces G2/M phase cell cycle arrest in liver cancer cells. a HepG2 and SMMC-7721 cells were treated with designated concentrations of OPE (0, 3.125, and 6.25 μg/mL) for 48 h and the cell cycle distribution was determined by a flow cytometric analysis. b Western blot analysis of protein expression levels of Cyclin D1, Cyclin A, Cyclin B1, and CDK1 in HepG2 and SMMC-7721 cells treated with OPE (0, 3.125, and 6.25 μg/mL) for 48 h. GAPDH was served as a loading control. c, d The relative protein expression of Cyclin D1, Cyclin A, Cyclin B1, and CDK1. Data are presented as mean ± SD of at least three independent experiments. (*p < 0.05; **p < 0.01;***p < 0.001, compared to untreated control)