Fig. 4
From: Ethanol extract of Ophiorrhiza pumila suppresses liver cancer cell proliferation and migration
OPE induces apoptosis in liver cancer cells. a HepG2 and SMMC-7721 cells were incubated with OPE at different doses for 48 h. The apoptosis rate was treated statistically. b Morphological features of nuclei of HepG2 and SMMC-7721 cells treated with various concentrations of OPE for 48 h (20 ×). c Western blot analyses of HepG2 and SMMC-7721 cells treated with OPE (0, 3.125, and 6.25 μg/mL) for 48 h to evaluate protein expression of Bax, Bcl-2, cleaved caspase-3. GAPDH was served as a loading control. d The relative protein expression of Bcl-2, Bax and cleaved caspase-3. e The percentage of Bax/Bcl-2 ratio. Data are presented as mean ± SD of at least three independent experiments. (*p < 0.05; ***p < 0.001, compared to untreated control)