Fig. 3

a H&E staining (in 10× and 40×) and b Nissl staining (in 20×) analysis of brain tissue in each group after reperfusion for 22 h. In H&E staining sections, neuronal pyramidal cell and its dendrite could be clearly observed, and central Nissl bodies were dissolved in red neurons. In Nissl staining, Nissl bodies were highlighted in dark blue. After modeling, neuronal cell degeneration and necrosis were observed and central Nissl bodies were dissolved, showing red neurons. c The number of Nissl bodies was analyzed by Image Pro Plus 6.0 software. Values are the mean ± SD (n = 6). Comparing model control group with sham surgery group, *P < 0.05; comparing treatment groups with model control group, ^#P < 0.05