Fig. 3

FAS, SREBP1-c and α-SMA protein expression level. Protein were extracted from livers and analysed by western blotting. Image J was used to do quantitative analysis. The lysate sample of each group was a mixture of 6 mice from the same group (n = 6 per group). The mixture sample of each group was prepared 3 times and the experiments were repeated at least 3 times. β-actin was used as a loading control a β-actin, FAS and SREBP1-c protein expression. b Quantity of FAS relative protein expression to β-actin. c Quantity of SREBP1-c relative protein expression to β-actin. d β-actin and α-SMA protein expression. e Quantity of α-SMA relative protein expression to β-actin. Data are expressed as mean ± SD. P value < 0.05 were considered significant. *P < 0.05 vs MCD diet, **P < 0.01 vs MCD diet, ***P < 0.05 vs MCD diet, #P < 0.05 vs MCS diet, ##P < 0.01vs MCD diet