UHPLC-QQQ-MS/MS assay for the quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: applications for the standardization of traditional Chinese medicines (TCMs) with endogenous toxicity

Table 3 Regression equation, LOD and LOQ of the six dianthrones

No.	Compounds	Regression equation	R²	Range (ng/mL)	LOD (ng/mL)	LOQ (ng/mL)
1	Polygonumnolide C4	Y = 10.186x + 0.0079	0.9980	2.1–126.0	0.4	1.1
2	Polygonumnolide C3	Y = 15.446x + 0.0163	0.9990	2.1–128.4	0.4	1.1
3	Polygonumnolide C1	Y = 17.122x + 0.0534	0.9985	2.8–169.8	0.3	1.1
4	Polygonumnolide C2	Y = 20.117x + 0.0466	0.9983	2.8–168.0	0.3	1.1
5	trans-Emodindianthrones	Y = 30.352x + 0.0800	0.9965	3.2–191.0	0.3	0.7
6	cis-Emodin dianthrones	Y = 33.308x + 0.0426	0.9978	2.8–168.2	0.3	0.7

ISSN: 1749-8546