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Table 5 Contents of 6 dianthrones in 90 batches of Polygoni Multiflori Radix (PMR)

From: UHPLC-QQQ-MS/MS assay for the quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: applications for the standardization of traditional Chinese medicines (TCMs) with endogenous toxicity

Sample no.

Contents of analytes (µg/g, n = 2a)

1

2

3

4

5

6

Total

Mean ± (SD%)b

Mean ± (SD%)b

Mean ± (SD%)b

Mean ± (SD%)b

Mean ± (SD%)b

Mean ± (SD%)b

PMR-01

2.23 ± 0.09

1.75 ± 0.19

1.81 ± 5.81

1.41 ± 0.73

6.94 ± 0.34

4.96 ± 14.32

19.10

PMR-02

0.84 ± 1.48

0.61 ± 3.34

0.69 ± 0.06

0.81 ± 2.58

1.71 ± 2.59

1.18 ± 1.31

5.84

PMR-03

0.60 ± 1.77

0.47 ± 0.98

0.43 ± 0.93

0.62 ± 3.85

1.22 ± 0.20

0.82 ± 4.54

4.16

PMR-04

19.08 ± 10.75

13.86 ± 11.51

15.53 ± 10.02

12.04 ± 13.86

45.33 ± 41.21

37.33 ± 20.60

143.17

PMR-05

0.83 ± 0.32

0.73 ± 0.63

0.67 ± 2.00

0.78 ± 1.07

6.90 ± 15.20

5.22 ± 16.45

15.13

PMR-06

4.62 ± 0.07

3.99 ± 1.43

4.15 ± 1.22

2.30 ± 0.84

10.37 ± 1.79

9.20 ± 0.35

34.63

PMR-07

0.70 ± 1.03

0.49 ± 0.62

0.56 ± 0.93

0.64 ± 1.93

1.38 ± 0.51

0.68 ± 2.72

4.45

PMR-08

1.43 ± 2.01

0.66 ± 0.36

1.06 ± 0.84

1.30 ± 2.94

4.94 ± 9.23

2.89 ± 4.05

12.28

PMR-09

1.87 ± 0.17

1.21 ± 0.21

1.28 ± 0.23

1.24 ± 2.54

1.55 ± 1.51

1.19 ± 3.36

8.34

PMR-10

3.78 ± 0.35

3.33 ± 0.46

3.57 ± 0.08

1.75 ± 1.60

9.07 ± 1.85

7.08 ± 0.77

28.58

PMR-11

1.52 ± 9.04

1.17 ± 3.03

1.35 ± 1.24

1.45 ± 7.84

8.42 ± 59.40

4.85 ± 5.31

18.76

PMR-12

2.24 ± 5.62

1.51 ± 2.21

1.76 ± 1.00

1.72 ± 2.55

6.51 ± 3.46

5.06 ± 4.21

18.80

PMR-13

2.38 ± 2.36

1.41 ± 0.74

1.64 ± 1.09

2.10 ± 2.36

9.36 ± 81.61

5.75 ± 18.09

22.64

PMR-14

2.56 ± 2.36

1.92 ± 3.47

2.04 ± 1.44

1.55 ± 2.20

3.78 ± 18.58

3.03 ± 10.02

14.88

PMR-15

2.52 ± 0.33

3.03 ± 0.74

2.78 ± 1.67

1.50 ± 0.01

10.64 ± 3.47

10.82 ± 2.61

31.29

PMR-16

7.80 ± 2.01

6.18 ± 2.48

6.49 ± 0.69

4.29 ± 0.92

27.53 ± 4.71

25.28 ± 5.37

77.57

PMR-17

0.14 ± 0.50

0.17 ± 0.34

0.16 ± 0.35

0.037 ± 0.16

1.01 ± 10.91

0.46 ± 1.36

1.98

PMR-18

0.25 ± 0.60

0.022 ± 0.00

0.073 ± 0.00

0.74 ± 0.03

1.01 ± 6.87

0.82 ± 1.96

2.92

PMR-19

0.69 ± 0.14

0.52 ± 0.16

0.33 ± 0.63

1.62 ± 2.82

7.31 ± 40.20

5.35 ± 14.37

15.82

PMR-20

5.91 ± 0.12

0.94 ± 0.54

0.69 ± 0.04

3.90 ± 0.17

64.18 ± 8.23

48.48 ± 3.40

124.1

PMR-21

0.20 ± 0.88

2.82 ± 0.33

5.10 ± 5.70

0.50 ± 0.74

4.15 ± 5.15

2.84 ± 0.01

15.61

PMR-22

0.027 ± 0.28

0.48 ± 0.75

0.27 ± 0.92

0.034 ± 0.72

0.47 ± 2.22

0.29 ± 2.25

1.57

PMR-23

0.042 ± 0.10

0.11 ± 0.71

0.10 ± 0.08

0.10 ± 0.43

0.63 ± 3.82

0.41 ± 0.62

1.39

PMR-24

1.87 ± 2.82

0.181 ± 0.33

0.13 ± 0.30

2.04 ± 0.61

2.54 ± 3.68

1.84 ± 0.45

8.60

PMR-25

2.58 ± 0.30

1.091 ± 0.00

1.42 ± 3.62

23.35 ± 19.62

18.06 ± 1.04

13.68 ± 2.07

60.18

PMR-26

0.55 ± 0.02

2.211 ± 0.02

2.63 ± 0.33

1.51 ± 0.12

22.66 ± 7.04

20.44 ± 0.09

50.00

PMR-27

0.42 ± 1.45

0.791 ± 0.10

1.18 ± 0.19

1.54 ± 2.72

8.24 ± 22.57

5.47 ± 18.23

17.64

PMR-28

0.48 ± 0.29

1.22 ± 4.70

0.57 ± 0.19

1.20 ± 0.37

7.93 ± 10.35

6.24 ± 16.38

17.64

PMR-29

0.054 ± 0.03

0.76 ± 1.43

0.54 ± 0.79

0.037 ± 0.39

4.17 ± 2.97

3.31 ± 2.33

8.87

PMR-30

0.27 ± 0.54

0.13 ± 0.53

0.11 ± 0.18

0.15 ± 0.42

1.03 ± 0.08

0.82 ± 1.30

2.51

PMR-31

5.20 ± 1.85

0.19 ± 0.06

0.27 ± 0.02

3.96 ± 0.21

10.74 ± 2.01

8.84 ± 3.84

29.20

PMR-32

1.27 ± 0.35

2.89 ± 3.50

3.22 ± 7.05

0.92 ± 2.08

5.12 ± 5.83

4.66 ± 6.80

18.08

PMR-33

6.83 ± 0.15

0.83 ± 0.97

0.95 ± 1.35

5.82 ± 2.78

51.11 ± 13.81

42.50 ± 3.46

108.04

PMR-34

4.89 ± 1.01

6.02 ± 0.36

6.52 ± 0.38

3.41 ± 2.06

21.71 ± 2.04

15.11 ± 4.99

57.66

PMR-35

7.67 ± 0.42

3.28 ± 0.48

4.34 ± 0.09

5.81 ± 0.60

61.94 ± 0.67

50.02 ± 5.90

133.06

PMR-36

3.38 ± 1.19

6.11 ± 2.08

7.37 ± 0.36

1.73 ± 0.82

16.51 ± 0.62

12.09 ± 0.64

47.19

PMR-37

0.54 ± 0.63

2.47 ± 0.08

3.28 ± 0.33

0.32 ± 2.23

2.12 ± 6.34

1.55 ± 2.14

10.28

PMR-38

4.17 ± 10.69

0.36 ± 1.36

0.48 ± 0.03

2.50 ± 1.15

8.77 ± 5.04

7.51 ± 0.86

23.79

PMR-39

2.01 ± 0.33

2.81 ± 8.05

3.23 ± 4.53

1.13 ± 3.14

5.79 ± 38.26

5.58 ± 11.65

20.55

PMR-40

1.56 ± 0.23

1.37 ± 1.06

1.56 ± 4.16

1.03 ± 0.20

7.07 ± 20.29

5.61 ± 9.30

18.20

PMR-41

6.02 ± 11.72

1.113 ± 0.38

1.32 ± 5.52

3.75 ± 1.79

7.71 ± 6.91

6.26 ± 21.43

26.17

PMR-42

4.68 ± 3.66

3.85 ± 5.45

4.47 ± 2.27

2.86 ± 1.09

6.45 ± 1.67

5.70 ± 23.76

28.01

PMR-43

10.39 ± 0.67

3.16 ± 2.64

3.58 ± 14.24

9.88 ± 0.86

27.34 ± 7.43

20.75 ± 0.12

75.10

PMR-44

1.25 ± 3.60

0.79 ± 3.24

0.66 ± 0.91

1.34 ± 2.87

2.51 ± 2.49

1.87 ± 4.10

8.42

PMR-45

0.92 ± 0.18

0.60 ± 1.06

0.59 ± 0.88

0.64 ± 1.18

4.68 ± 1.77

3.27 ± 17.57

10.7

PMR-46

1.28 ± 0.74

0.83 ± 1.20

0.74 ± 1.03

1.12 ± 0.98

2.35 ± 4.83

1.68 ± 3.90

8.00

PMR-47

2.26 ± 1.93

1.34 ± 3.33

1.35 ± 0.94

1.71 ± 1.61

9.27 ± 16.24

6.60 ± 6.12

22.53

PMR-48

1.45 ± 2.29

0.88 ± 1.09

0.91 ± 0.03

0.72 ± 3.24

7.18 ± 22.72

6.43 ± 31.68

17.57

PMR-49

1.70 ± 0.85

0.93 ± 0.33

1.02 ± 0.67

1.14 ± 2.18

6.03 ± 0.71

4.94 ± 13.15

15.76

PMR-50

0.94 ± 0.95

0.53 ± 1.70

0.55 ± 0.99

0.64 ± 0.69

4.48 ± 21.93

3.16 ± 15.33

10.3

PMR-51

1.22 ± 1.04

0.65 ± 1.13

0.76 ± 1.04

0.80 ± 2.97

6.91 ± 26.37

6.53 ± 28.27

16.87

PMR-52

1.30 ± 4.47

0.80 ± 1.93

0.74 ± 3.43

0.74 ± 4.23

3.35 ± 18.15

2.60 ± 17.49

9.53

PMR-53

7.62 ± 0.08

0.69 ± 0.19

5.63 ± 0.90

7.22 ± 0.34

47.24 ± 6.47

38.91 ± 5.55

107.31

PMR-54

3.01 ± 0.17

3.30 ± 0.17

2.42 ± 1.11

2.85 ± 0.93

38.15 ± 9.29

33.72 ± 1.68

83.45

PMR-55

6.76 ± 7.02

4.92 ± 7.27

3.93 ± 4.57

5.17 ± 5.17

83.67 ± 3.75

67.00 ± 9.16

171.45

PMR-56

0.32 ± 0.51

0.22 ± 1.18

0.17 ± 0.14

0.28 ± 1.06

6.78 ± 28.19

4.95 ± 6.74

12.72

PMR-57

0.61 ± 0.50

0.35 ± 0.20

0.37 ± 0.81

0.42 ± 0.51

1.59 ± 0.79

1.35 ± 0.16

4.69

PMR-58

1.25 ± 1.12

0.92 ± 0.54

0.81 ± 2.83

0.95 ± 0.48

9.33 ± 11.15

6.29 ± 37.35

19.55

PMR-59

1.29 ± 0.21

0.90 ± 3.74

0.85 ± 0.82

0.91 ± 1.22

9.48 ± 9.83

6.30 ± 15.03

19.73

PMR-60

1.46 ± 0.75

1.09 ± 2.17

0.96 ± 1.72

1.03 ± 3.02

9.51 ± 5.22

6.47 ± 4.66

20.52

PMR-61

3.99 ± 1.92

2.63 ± 2.76

2.30 ± 4.61

2.31 ± 0.92

12.10 ± 15.76

9.72 ± 19.35

33.05

PMR-62

1.77 ± 4.12

1.01 ± 0.31

1.03 ± 2.95

1.15 ± 1.08

4.55 ± 12.20

3.83 ± 8.55

13.34

PMR-63

0.82 ± 1.08

0.81 ± 0.66

0.54 ± 1.22

0.80 ± 0.91

5.39 ± 1.52

4.24 ± 5.97

12.6

PMR-64

0.46 ± 0.91

0.29 ± 0.98

0.27 ± 0.13

0.31 ± 0.08

1.42 ± 1.13

1.40 ± 0.53

4.15

PMR-65

1.41 ± 0.36

0.94 ± 0.51

1.02 ± 0.07

1.37 ± 0.21

7.01 ± 0.84

6.76 ± 3.08

18.51

PMR-66

1.16 ± 1.65

0.72 ± 0.30

0.71 ± 0.35

0.74 ± 2.46

3.84 ± 5.18

3.34 ± 9.40

10.51

PMR-67

1.47 ± 2.11

0.99 ± 0.30

0.91 ± 2.47

0.82 ± 0.98

7.48 ± 22.19

6.91 ± 12.61

18.58

PMR-68

1.60 ± 0.24

0.97 ± 1.97

0.92 ± 6.10

1.01 ± 2.62

2.57 ± 11.58

2.03 ± 13.81

9.10

PMR-69

0.77 ± 0.25

0.51 ± 0.16

0.52 ± 0.14

0.78 ± 0.34

8.49 ± 2.12

8.84 ± 0.66

19.91

PMR-70

0.38 ± 0.35

0.16 ± 0.51

0.18 ± 0.64

0.39 ± 0.26

3.46 ± 9.85

2.66 ± 2.92

7.23

PMR-71

1.11 ± 5.67

0.71 ± 4.43

0.67 ± 1.40

0.74 ± 5.16

3.50 ± 19.82

2.38 ± 21.85

9.11

PMR-72

0.96 ± 2.83

0.80 ± 2.48

0.68 ± 0.03

0.72 ± 3.11

7.88 ± 15.41

5.45 ± 6.51

16.49

PMR-73

0.75 ± 0.27

0.41 ± 0.64

0.37 ± 0.99

0.79 ± 0.33

3.37 ± 7.62

2.49 ± 7.62

8.18

PMR-74

2.63 ± 0.78

0.97 ± 1.51

1.46 ± 0.24

2.98 ± 0.64

19.43 ± 1.13

14.40 ± 2.76

41.87

PMR-75

2.34 ± 0.29

1.42 ± 2.21

0.91 ± 1.94

2.22 ± 0.21

66.05 ± 13.61

41.98 ± 3.82

114.92

PMR-76

0.25 ± 0.24

0.12 ± 1.35

0.15 ± 0.11

0.23 ± 0.18

1.26 ± 2.63

0.83 ± 0.57

2.84

PMR-77

0.46 ± 0.35

0.39 ± 1.12

0.25 ± 0.36

0.40 ± 1.89

3.92 ± 0.17

3.14 ± 2.26

8.56

PMR-78

1.14 ± 1.65

0.74 ± 2.18

0.66 ± 0.99

0.70 ± 1.99

3.03 ± 15.82

2.38 ± 17.31

8.65

PMR-79

0.61 ± 4.10

0.49 ± 4.96

0.34 ± 4.95

0.40 ± 0.26

5.46 ± 11.44

3.59 ± 9.55

10.89

PMR-80

0.26 ± 0.66

0.15 ± 0.62

0.12 ± 0.07

0.27 ± 0.10

0.38 ± 0.31

0.37 ± 0.88

1.55

PMR-81

0.67 ± 0.17

0.35 ± 0.60

0.45 ± 0.13

0.92 ± 0.24

8.68 ± 3.51

7.25 ± 3.26

18.32

PMR-82

4.59 ± 0.34

3.78 ± 1.03

3.21 ± 1.38

4.14 ± 0.94

42.84 ± 8.21

37.40 ± 1.94

95.96

PMR-83

0.18 ± 2.23

0.089 ± 1.91

0.10 ± 1.67

0.14 ± 0.07

1.47 ± 1.69

0.94 ± 0.42

2.919

PMR-84

2.28 ± 0.34

1.15 ± 1.18

1.35 ± 0.89

1.95 ± 0.26

11.61 ± 11.10

9.29 ± 7.78

27.63

PMR-85

0.84 ± 0.09

0.47 ± 0.14

0.51 ± 0.87

0.82 ± 0.39

12.74 ± 2.17

12.40 ± 1.07

27.78

PMR-86

2.06 ± 1.19

1.31 ± 3.95

1.22 ± 3.72

1.33 ± 5.86

3.97 ± 21.77

3.47 ± 18.51

13.36

PMR-87

6.11 ± 0.10

5.20 ± 1.19

4.61 ± 0.93

4.82 ± 0.22

52.09 ± 15.47

46.51 ± 7.87

119.34

PMR-88

0.89 ± 6.61

0.53 ± 2.83

0.50 ± 5.50

0.52 ± 1.83

3.23 ± 5.44

1.99 ± 0.73

7.66

PMR-89

2.13 ± 1.71

1.35 ± 0.48

1.22 ± 5.08

1.28 ± 1.00

4.92 ± 1.91

3.69 ± 4.18

14.59

PMR-90

0.98 ± 0.36

0.65 ± 2.25

0.59 ± 1.27

0.60 ± 1.91

4.44 ± 17.23

3.93 ± 19.42

11.19

Average

2.30

1.53

1.67

1.99

12.23

9.75

29.46

  1. aThe data are presented as the average of two replicates
  2. bSD% is presented in the table