From: Traditional application and modern pharmacological research of Eucommia ulmoides Oliv.
Compounds | Subjects | Dose | Effects | Notes | References |
---|---|---|---|---|---|
Methanol extract of E. ulmoides leaf (MEEuL) | False aged model rats | 1.8 and 2.7Â g of dried E. ulmoides leaf/kg | MEEuL promoted collagen synthesis in a dose-dependent manner (increase of 68% and 131% respectively) | These fractions were fractioned with organic reagent sequentially | [28] |
N-hexane fraction of the methanol extract of leaf (NF-MEEuL) | Â | 1.5 and 3.1Â g of dried E. ulmoides leaf/kg | No significant effect on collagen synthesis | Â | Â |
AcOEt fraction of the methanol extract of leaf (AEF-MEEuL) | Â | 1.5 and 2.9Â g of dried E. ulmoides leaf/kg | No significant effect on collagen synthesis | Â | Â |
Acetone fraction of the methanol extract of leaf (AF-MEEuL) | Â | 1.5 and 3.0Â g of dried E. ulmoides leaf/kg | AF-MEEuL promoted collagen synthesis in a dose-dependent manner (increase of 57% at dose of 3.0Â g of dried E. ulmoides leaf/kg) | Â | Â |
Methonal fraction of the methanol extract of leaf (MF-MEEuL) | Â | 1.5 and 3.0Â g of dried E. ulmoides leaf/kg | No significant effect on collagen synthesis | Â | Â |
Geniposidic acid | Â | 25, 50 and 100Â mg/kg | Geniposidic acid promoted collagen synthesis in a dose-dependent manner (increase of 79%, 82%, 95% respectively) | Â | Â |
Aucubin | Â | 25 and 50Â mg/kg | Aucubin promoted collagen synthesis in a dose-dependent manner (increase of 99% at dose of 50Â mg/kg) | Â | Â |
Methanol extract of E. ulmoides cortex (MEEuC) | Osteoblast, MG- 63 and Saos-2 (osteoblast-like cells); mouse bone marrow cells and ST-2 cells (osteoclast-like cells) | 1 mg/ml in GH inducing test; 1 × 10 −1–1 × 10 −11 mg/ml in MTT test and ALP activity assay; 1 × 10 −1–1 × 10 −7 mg/ml in nhibition of osteoclast | MEEuC showed potent induction of growth hormone (GH) release in the same extent as GH releasing factors (GRF) 1.0 mMMEEuC promoted cell proliferation and differentiation of osteoblast 32% at 1 × 10 −8 mg/ml MEEuC promoted maturation of osteoblast 10% at 1 × 10 −1 mg/ml. MEEuC inhibited around 35% of osteoclast at 1 × 10 −3–1 × 10 −5 mg/ml | MTT assay shows the proliferation and differentiation of osteoblast using osteoblast-like cells. ALP activity detection assays the activity of ALP, a specific enzyme relating to the maturation step of osteoblast Praline incorporation test shows the effects on the synthesis of collagen participating in the matrix formation of osteoblast | [30] |
N-hexane fraction of the methanol extract of cortex (NF-MEEuC) |  | Each fractions: 20 µg/ml in GH inducing test; 1 × 10 −1–1 × 10 −11 mg/ml in MTT test and ALP activity assay; 1 × 10 −2–1 × 10 −6 mg/ml in Praline incorporation test | NF-MEEuC showed potent induction of GH release in the same extent as GRF 0.5 mM |  | |
Chloroform fraction of the methanol extract of cortex (CF-MEEuC) |  | CF-MEEuC showed potent induction of GH release in the same extent as GRF 0.5 mM CF-MEEuC promoted maturation of osteoblast 38% at 1 × 10 −6 mg/ml CF-MEEuC promoted synthesis of collagen participating in the matrix formation of osteoblast 40% at 1 × 10 −3 mg/ml |  |  | |
Ethylacetate fraction of the methanol extract of cortex (EF-MEE) |  |  | EF-MEE showed potent induction of GH release in the same extent as GRF 0.5 mM EF-MEE promoted synthesis of collagen participating in the matrix formation of osteoblast 20% at 1 × 10 −5 mg/ml |  |  |
Butanol fraction of the methanol extract of cortex (BF-MEEuC) |  |  | BF-MEEuC promoted cell proliferation and differentiation of osteoblast 38% at 1 × 10 −1 mg/ml |  |  |
Aqueous fraction of the methanol extract of cortex (AF-MEEuC) |  |  | AF-MEEuC promoted cell proliferation and differentiation of osteoblast 26% at 1 × 10 −6 mg/ml |  |  |
Geniposidic acid |  | 10 µg/ml in GH inducing test; 1 × 10 −1–1 × 10 −11 mg/ml in MTT test and ALP activity assay; 1 × 10 −2–1 × 10 −6 mg/ml in Praline incorporation test; 1 × 10 −1–1 × 10 −7 mg/ml in inhibition of osteoclast | Geniposidic acid inhibited activities of osteoclast in dose-dependent manner (IC50 at 4.43 × 10 −7 M) |  |  |
Geniposide |  | Geniposide promoted cell proliferation and differentiation of osteoblast 35% at 1 × 10 −4 mg/ml. Geniposide promoted synthesis of collagen participating in the matrix formation of osteoblast 20% at 1 × 10 − 3–1 × 10 −5 mg/ml |  |  | |
Aucubin |  | Aucubin promoted cell proliferation and differentiation of osteoblast 34% at 1 × 10 −4 mg/ml Aucubin promoted maturation of osteoblast 29% at 1 × 10 −11 mg/ml. Aucubin inhibited activities of osteoclast in dose-dependent manner (inhibited 40% at 1 × 10 −3 mg/ml) |  |  |