Fig. 2

HGWD attenuated mechanical allodynia, thermal hyperalgesia, and morphological changes of DRG in PIPN rats. a Protocol showing drug administration and behavioral tests in the rat model. b Mechanical withdrawal threshold was measured by the von Frey test. c Thermal withdrawal threshold was evaluated by the hot plate test. d Time course effect of HGWD on body weight of rats. e DRG sections were stained by Nissl and observed by light micrographs (80 ×). The occurrence of eccentric nucleoli (red arrow) and multinucleolated neurons (red arrowhead) were analyzed. Histograms show the effect of HGWD on the incidence of nuclear and nucleolar pathological features. ^*P < 0.05, ^**P < 0.01 ^***P < 0.001, vs. vehicle group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs. PIPN group, n = 8 rats/group