Fig. 4

WMW alleviates TNBS-induced colitis by inhibiting necroptosis. A Representative TUNEL staining of different groups. Scale bar, 50 μm. (n = 4–6). B Representative western blots for caspase-8, p-RIPK1, RIPK1, p-RIPK3, RIPK3, p-MLKL and MLKL protein expressions in colon, and the quantification of caspase-8, p-RIPK1, p-RIPK3 and p-MLKL western blots. (n = 6). C Representative western blots for caspase-3 expression in colon, and the quantification of cleaved caspase-3 western blots. (n = 6). All data are presented as means ± SEM. *p < 0.05, ** p < 0.01, ***p < 0.001