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Table 3 The stability test results in enzymolysis matrix under various storage conditions (n = 5)

From: UPLC-MS/MS method for simultaneously determining nucleosides and methyl-nucleosides in liver mRNA of Epimedin C-induced liver injury mouse model

Analytes Added (pg/mL) Room temperature for 6 h Autosampler for 20 h (RT) Three freeze–thaw cycles Freezing for 30 days (− 20℃)
Measured (pg/mL) RE (%) Measured (pg/mL) RE (%) Measured (pg/mL) RE (%) Measured (pg/mL) RE (%)
Adenosine 120 130.0 ± 2.1 8.3 133.0 ± 1.0 10.8 134.2 ± 3.6 11.8 133.0 ± 1.6 10.8
16,000 15,360.0 ± 230.2 − 4.0 15,380.0 ± 164.3 − 3.9 15,240.0 ± 54.8 − 4.8 14,840.0 ± 167.3 − 7.3
Uridine 6000 6316.0 ± 170.7 5.3 6052.0 ± 169.9 0.9 5986.0 ± 141.2 − 0.2 5868.0 ± 140.2 − 2.2
800,000 706,000.0 ± 12,308.5 − 11.8 688,800.0 ± 6760.2 − 13.9 687,200.0 ± 15,073.2 − 14.1 716,600.0 ± 13,794.9 − 10.4
Cytidine 120 120.8 ± 2.9 0.7 121.0 ± 1.9 0.8 119.0 ± 4.4 − 0.8 117.2 ± 4.1 − 2.3
16,000 16,140.0 ± 240.8 0.9 16,040.0 ± 230.2 0.3 16,080.0 ± 238.7 0.5 15,980.0 ± 228.0 − 0.1
Guanosine 600 653.0 ± 16.3 8.8 625.8 ± 14.8 4.3 628.2 ± 14.8 4.7 605.2 ± 28.1 0.9
80,000 79,200.0 ± 748.3 − 1.0 77,520.0 ± 1158.4 − 3.1 76,300.0 ± 1647.7 − 4.6 77,320.0 ± 2008.0 − 3.4
m6A 120 132.4 ± 3.0 10.3 126.0 ± 3.4 5.0 123.8 ± 3.0 3.2 132.2 ± 12.1 10.2
16,000 15,962.0 ± 205.7 − 0.2 15,561.0 ± 218.9 − 2.7 15,490.8 ± 269.4 − 3.2 15,841.8 ± 281.8 − 1.0
m5C 120 123.4 ± 3.8 2.8 121.6 ± 2.2 1.3 120.0 ± 2.2 0 122.2 ± 3.7 1.8
16,000 15,720.0 ± 130.4 − 1.8 15,600.0 ± 100.0 − 2.5 15,600.0 ± 308.2 − 2.5 15,980.0 ± 249.0 − 0.1