Table 3 The stability test results in enzymolysis matrix under various storage conditions (n = 5)
Analytes | Added (pg/mL) | Room temperature for 6 h | Autosampler for 20 h (RT) | Three freeze–thaw cycles | Freezing for 30 days (− 20℃) | ||||
|---|---|---|---|---|---|---|---|---|---|
Measured (pg/mL) | RE (%) | Measured (pg/mL) | RE (%) | Measured (pg/mL) | RE (%) | Measured (pg/mL) | RE (%) | ||
Adenosine | 120 | 130.0 ± 2.1 | 8.3 | 133.0 ± 1.0 | 10.8 | 134.2 ± 3.6 | 11.8 | 133.0 ± 1.6 | 10.8 |
16,000 | 15,360.0 ± 230.2 | − 4.0 | 15,380.0 ± 164.3 | − 3.9 | 15,240.0 ± 54.8 | − 4.8 | 14,840.0 ± 167.3 | − 7.3 | |
Uridine | 6000 | 6316.0 ± 170.7 | 5.3 | 6052.0 ± 169.9 | 0.9 | 5986.0 ± 141.2 | − 0.2 | 5868.0 ± 140.2 | − 2.2 |
800,000 | 706,000.0 ± 12,308.5 | − 11.8 | 688,800.0 ± 6760.2 | − 13.9 | 687,200.0 ± 15,073.2 | − 14.1 | 716,600.0 ± 13,794.9 | − 10.4 | |
Cytidine | 120 | 120.8 ± 2.9 | 0.7 | 121.0 ± 1.9 | 0.8 | 119.0 ± 4.4 | − 0.8 | 117.2 ± 4.1 | − 2.3 |
16,000 | 16,140.0 ± 240.8 | 0.9 | 16,040.0 ± 230.2 | 0.3 | 16,080.0 ± 238.7 | 0.5 | 15,980.0 ± 228.0 | − 0.1 | |
Guanosine | 600 | 653.0 ± 16.3 | 8.8 | 625.8 ± 14.8 | 4.3 | 628.2 ± 14.8 | 4.7 | 605.2 ± 28.1 | 0.9 |
80,000 | 79,200.0 ± 748.3 | − 1.0 | 77,520.0 ± 1158.4 | − 3.1 | 76,300.0 ± 1647.7 | − 4.6 | 77,320.0 ± 2008.0 | − 3.4 | |
m6A | 120 | 132.4 ± 3.0 | 10.3 | 126.0 ± 3.4 | 5.0 | 123.8 ± 3.0 | 3.2 | 132.2 ± 12.1 | 10.2 |
16,000 | 15,962.0 ± 205.7 | − 0.2 | 15,561.0 ± 218.9 | − 2.7 | 15,490.8 ± 269.4 | − 3.2 | 15,841.8 ± 281.8 | − 1.0 | |
m5C | 120 | 123.4 ± 3.8 | 2.8 | 121.6 ± 2.2 | 1.3 | 120.0 ± 2.2 | 0 | 122.2 ± 3.7 | 1.8 |
16,000 | 15,720.0 ± 130.4 | − 1.8 | 15,600.0 ± 100.0 | − 2.5 | 15,600.0 ± 308.2 | − 2.5 | 15,980.0 ± 249.0 | − 0.1 | |