Fig. 1

Scheme for the study of potential mechanism of ZYTLF in the treatment of PMOP via network pharmacology and ovariectomized rat model. In this study, OVX rat model was established and administrated with ZYTLF. The result showed that ZYTLF can significantly improve bone mineral density and bone microstructure of OVX rats, confirmed by ALP + TRACP staining that ZYTLF can promote osteogenic activity and inhibit osteoclast activity. Furthermore, the network pharmacology technique was applied to predict the active components and potential targets of ZYTLF against PMOP. Finally, molecular docking technology was utilized to perform preliminary validation computationally. WB analysis on bone tissue of OVX rats was used to validate the mechanism of ZYTLF against PMOP