Fig. 2

The siRNA transfection to silence the expression of GAP43. a PC12 cells and primary cortical neurons were transfected by CY3 (red) labeled GAP43-si then visualized by fluorescent microscope. b The relative mRNA expression changes of GAP43 in neurons before and after Scu administration detected by qRT-PCR. c The relative mRNA levels of GAP43 after transfection in the normal, reagent, NC, F1, F2 groups detected by qRT-PCR. d The relative mRNA expression variation of GAP43 after interference in the normal, Reagent, NC and GAP43i groups detected by qRT-PCR. The cells in the reagent group were treated with transfection reagents (without siRNA), and the cells in the NC group were treated with an irregular small RNA consistent with the sequence base number of RNAi but random sequence. OGD Oxygen–glucose deprivation, RT-qPCR real-Time quantified polymerase chain reaction, Scu Scutellarin, NC negative control, F1 Fragment 1, F2 Fragment 2, GAP43i GAP43 silencing. Data are presented as the mean ± SD. *p < 0.01, p < 0.001, scale bar = 50 μm, n = 3/group