Fig. 2
From: Aloin induced apoptosis by enhancing autophagic flux through the PI3K/AKT axis in osteosarcoma
Aloin induced protein expression level changes in HOS and MG63 cell lines in vitro. The expression levels of apoptosis-related markers (A), autophagy-related markers (B) and PI3K/AKT/mTOR axis proteins (C) were determined by western blot after aloin administration (0, 100, and 300 μM) for 24 h. Western blot quantitative analysis was performed using ImageJ (ver 1.8). Error bar = mean ± SD of at least triplicate experiments. (*P < 0.005, **P < 0.01, ***P < 0.001, ****P < 0.0001)