Table 2 Sequences of the primers used in RT-qPCR to determine the mRNA expression of proinflammatory factors in a rat model of CIA
Gene | 5′–3′ | Sequence |
|---|---|---|
GAPDH | Forward | CCACCCATGGCAAATTCCATGGCA |
Reverse | TCTAGACGGCAGGTCAGGTCCACC | |
TNF-α | Forward | GGAAAGCATGATCCGAGATG |
Reverse | CGAGCAGGAATGAGAAGAGG | |
TRAF-6 | Forward | TCTCGGAGTGCTGCGTGTATAGG |
Reverse | GTCGCTTAGAGACTGGCTGGAC | |
IL-1β | Forward | CTCACAGCATCTCGACAAGAG |
Reverse | CACACTAGCAGGTCGTCATCATCC | |
IL-17 | Forward | GCCGAGGCCAATAACTTTCT |
Reverse | GAGTCCAGGGTGAAGTGGAA | |
CTSK | Forward | TAGCACCCTTAGTCTTCCGC |
Reverse | CTTGAACACCCACATCCTGC | |
NFATc-1 | Forward | GGAGAGTCCGAGAATCGAGAT |
Reverse | TTGCAGCTAGGAAGTACGTCT | |
TRAP | Forward | TGGGTGACCTGGGATGGATT |
Reverse | AGCCACAAATCTCAGGGTGG | |
c-Fos | Forward | TTTCAACGCCGACTACGAGG |
Reverse | GCGCAAAAGTCCTGTGTGTT | |
RANKL | Forward | AGGCTGGGCCAAGATCTCTA |
Reverse | GATAGTCCGCAGGTACGCTC | |
VEGF | Forward | GGCAGCTTGAGTTAAACGAAC |
Reverse | TGGTGACATGGTTAATCGGTC | |
HIF-1 | Forward | GGAAATGCTGGCTCC CTAT |
Reverse | CTGTAACTGGGTCTGCTGGA | |
TIMP-1 | Forward | CGAGACCACCTTATACCAGCG |
Reverse | ATGACTGGGGTGTAGGCGTA | |
OPG | Forward | GGCAGGGCATACTTCC TGTT |
Reverse | GCCACTTGTTCATTGTGGTCC |