Fig. 3

HK alleviates lipid accumulation through SIRT3-AMPK-induced autophagy. P/O-stimulated AML12 cells were treated without or with various concentrations of HK for 24 h. A p-AMPK and AMPK protein levels evaluated by Western blotting. GAPDH was used as a loading control. B P/O-stimulated AML12 cells were treated without or with HK (10 μM) and CC (10 μM) for 24 h. p-AMPK, AMPK, Beclin1, Atg5, p62 and LC3 protein levels were detected by Western blotting. GAPDH was used as a loading control. C The lipid content determined by Nile red staining. D The cellular TG content. Data represented means ± SD. **p < 0.01 and ***p < 0.001, HK vs. P/O treatment. ##p < 0.001, CC vs. P/O treatment. One-way ANOVA was used to calculate the p-values