Fig. 3

BHD reduces renal fibrosis and inflammation in STZ-induced diabetic mice. A Masson’s Trichrome staining and semi-quantification. Extracellular matrix deposition is shown in blue. B Immunohistochemistry of F4/80-positive cells showing macrophage infiltration in glomeruli and tubulointerstitium. DM, diabetes mellitus. Irbesartan (IRB) was taken as a positive control. Data represent the means  ±  SEM for groups of six animals. Scale bar: 50 µm. *P  <  0.05, **P  <  0.01, ***P  <  0.001 versus normal mice. ^#P  <  0.05, ^##P  <  0.01, ^###P  <  0.001 versus DM mice