Fig. 2

Honokiol activates Nrf2/HO-1 and improves the oxidative stress in BEAS-2B cells. BEAS-2B cells were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min in the presence or absence of HKL. A, B The protein expression of Total-Nrf2, Nul-Nrf2 and HO-1 was measured by western blot. C The mRNA expression of Nrf2 and HO-1 was measured by RT-PCR. D–F The levels of MPO, MDA and SOD in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01 compared with the control group, ^#P < 0.05, ^##P < 0.01 compared with LPS + ATP group