Fig. 5

Representative Western blotting of pGluA1ser845 and pGluA1ser831 from hippocampus of mice treated with saline, desipramine (20 mg/kg) or echinacoside (20, 30 or 40 mg/kg) (A), after echinacoside (30 mg/kg) administration with pretreatment with NBQX (30 mg/kg) or rapamycin (20 mg/kg) (D) and after echinacoside (30 mg/kg) administration with pretreatment with SL327 (40 mg/kg) or MK2206 (60 mg/kg) (G). Echinacoside at 30 mg/kg treatment significantly increases the expression of pGluA1ser845 (B) and pGluA1ser831 (C), which were not observed in mice with other treatments. The densitometry analyses of the blot (normalized to β-actin) verify the enhanced activities of pGluA1ser845 (E), and pGluA1ser831 (F) in the echinacoside at 30 mg/kg administrated group and decreased activities in the NBQX, but, not in rapamycin pretreated groups (D–F). The up-regulations of pGluA1ser845 and pGluA1ser831 were not blocked by pretreatment of SL327 or MK2206 (G–I). n = 4 each group; *p < 0.05, Mann–Whitney U test; Values shown are mean ± SEM