Fig. 8

Identify the critical component of LGZG that inhibit the activation of STING pathway in liver macrophages. a, b Concentration of TNFα (a) and IFNβ (b) from the culture supernatant of liver macrophages treated with DMXAA and DMXAA co-cultured with 10^–3 g/ml LGZG (LGZG-H), 60 μM Poriaic acid (PA60), 40 μM Cinnamaldehyde (CA40), 50 μM Atractylenolide II(ATR50), 250 μM Glycyrrhizinate(GLY250) and 40 μM mixture of 4 components of LGZG(MIX40). c, d Immunoblotting analysis of the expression of NF-κB, pNF-κB, TBK1, p-TBK1 and STING. Quantification of pNF-κB/NF-κB levels (d) p-TBK1/TBK1 levels (e). The data are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001 vs. DMXAA group (Model)