Fig. 6

Wnt5 or β-Catenin inhibitor promotes the inhibition ability of AR-CS in proliferation and migration of SW620 cells. SW620 cells were treated with or without 20% AR-CS, Wnt5 inhibitor (IWP-4), and β-Catenin inhibitor (JW55) treated for 24 h, real-time qPCR was used to detect Wnt5 (A), β-Catenin (B), ARF6 (C), and N-Cadherin (D) mRNA expression. Western blot was used to detect Wnt5, β-Catenin (E), ARF6, and N-Cadherin (F) protein expression. Wnt5 (G), β-Catenin (H), ARF6 (I), and N-Cadherin (J) protein expression level was normalized to control group. Transwell assay (K) was used to detect cell migration rate (L). Wound scratch assay (M) was used to detect cell wound healing rate (N). Immunofluorescent staining was used to detect β-Catenin expression (O) and the fluorescence density was calculated (P). Scale bar = 100 µm in K, Scale bar = 400 µm in M, Scale bar = 20 µm in O. N = 6, in indicating comparison, *p < 0.05