Fig. 4

Cell morphology was observed at 400 magnification and EGb on EMT of HK-2 cells treated by cisplatin. a HK-2 group; b Model group; c EGb group; d SIS3 group; e EGb  +  SIS3 group. Representative immunohistochemical images of α-SMA (B), E-cadherin (D), Vimentin (F) in different groups of HK-2 cells. Nuclei were counterstained with DAPI (blue). Scale bar: 200 μm. Quantitative analysis of C α-SMA, E E-cadherin and G Vimentin expression in HK-2 cells. H Western blot analysis of α-SMA, vimentin, COL-I, E-cadherin, TIMP-1, MMP-1, CTGF expression in HK-2 cells. Quantitative analysis of the levels of I α-SMA, J vimentin, K COL-I, L E-cadherin, M TIMP-1, N MMP-1/TIMP-1, O CTGF in HK-2 cells. Reverse transcription-quantitative PCR analysis P α-SMA and Q vimentin mRNA expression in HK-2 cells. n  = 3. *P  < 0.05 vs. HK-2; ^#P  < 0.05 vs. Model; ^&P  < 0.05 vs. SIS3