Fig. 5

The effect of EGb on the protein levels of TGF-β1, p-smad2/3, smad2/3, p-p38MAPK and p38 MAPK, and TGF-β1 mRNA level in HK-2 cells. Representative images of immunofluorescence staining of A TGF-β1, C p-smad2/3 in different groups of HK-2 cells. Nuclei were counterstained with DAPI (blue). Scale bar: 200 μm. Quantification of the protein levels of B TGF-β1, D p-smad2/3 in HK-2 cells. E Western blot analysis of smad2/3, p-p38 MAPK, p38 MAPK and TGF-β1 expression in HK-2 cells. Quantitative analysis the protein levels of F TGF-β1, G, H smad2/3, I p-p38 MAPK and J p38 MAPK in HK-2 cells. Reverse transcription-quantitative PCR analysis of TGF-β1 mRNA expression K in HK-2 cells. n  = 3. *P  < 0.05 vs. HK-2; ^#P  < 0.05 vs. Model; ^&P  < 0.05 vs. SIS3