Table 2 Primer sequences for qRT-PCR
Gene | Primer sequences (5′→3′) | Product (bp) | Accession no. |
|---|---|---|---|
GAPDH | Forward: TGTGGATCAGCAAGCAGGAG Reverse: ACGCAGCTCAGTAACAGTCC | 87 | NM_001289726.1 |
AQP1 | Forward: TTCCTGGTCTCAGAGCTTCC Reverse: TGGGTCCCTCACTTTCACTC | 91 | NM_007472.2 |
AQP3 | Forward: CTTGTGATGTTTGGCTGTGG Reverse: AAGCCAAGTTGATGGTGAGG | 85 | NM_016689.2 |
AQP4 | Forward: GTGTCTGTGGCAGCGAGATA Reverse: GCATCTGCCTCAGAACATGA | 116 | NM_001308641.1 |
ZO-1 | Forward: TTCAGCAGCAACAGAACCAG Reverse: CGATCGTCATGCAAATCAAG | 88 | XM_006540786.4 |
Occludin | Forward: GAATGGCAAGCGATCATACC Reverse: CTGCCTGAAGTCATCCACAC | 106 | NM_001360536.1 |