Fig. 6

TOD attenuates the activation of the NF-κB and MAPK signalling pathways in BMMs. A Western blotting performed the activity of NF-κB and MAPK signalling pathways in RAW264.7 cells; GAPDH was used as the loading control. B ImageJ software was used to quantify the density of the western blot bands (P-P65, IκBα, P-P38, and P-JNK) shown in A. C Western blots performed the protein expression of P65 in the nucleus and cytoplasm in RAW264.7 cells. D ImageJ software was used to quantify the density of the western blot bands in C. E Immunofluorescence was used to determine nuclear translocation of p65 in RAW264.7 cells. Scale bar: 100 μm. Data represent means ± SD of triplicate independent experiments. ^{*, #} indicates p < 0.05, ^{**, ##} indicates p < 0.01, ^ns indicates not significant