Fig. 3

CR inhibited CC cells growth and decreased its viability. A CCK-8 assay was performed to measure cell viability at 24 h and 48 h after CR treatment at different dosages (0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 mg/mL). B CCK-8 assay was performed to measure cell growth ability at 24 h and 48 h after CR treatment at 24 h IC₅₀ (0.27 mg/mL and 0.12 mg/mL) in HT-29 and HCT-15 cells, respectively. C Cell colony formation assay was performed to measure cell clonality after CR treatment for 24 h. ***p < 0.001 and ****p < 0.0001 based on the Student's t-test. D The Operetta CLS High Content Analysis System was used to evaluate the accumulated distance of CC cells at 24 h after CR treatment. **p < 0.01 and ***p < 0.001 based on the Student's t-test