Fig. 2

AGNHW treatment decreased mortality rates and improved neurological deficit in transient ischemic stroke treated with delayed t-PA. Sham, sham operation with no MCA ligation; M5/R19, 5 h of MCAO plus 19 h of reperfusion with saline as vehicle treatment (1 ml). M5/R19 + t-PA, rats were treated with t-PA infusion (10 mg/kg) for half an hour started from 4.5 h after MCAO, and followed by 19 h of reperfusion; M5/R19 + t-PA + AGNHW, rats were subjected to M5/R19 + t-PA (10 mg/kg dissolved in 1 ml saline) with the treatment of AGNHW (257 mg/kg) orally at 2 h after MCAO. A Diagram summarizing the design of the animal study. B Mortality rates: Mortality was calculated at 24 h after MCAO operations. Delayed t-PA treatment increased the mortality rate in the M5/R19 rats which was mitigated by AGNHW treatment. C Neurological deficit scores: The mNSS was used to account for neurological deficit scores at 24 h after MCAO operations. The M5/R19 + t-PA rats had increased the mNSS value which was reduced by AGNHW treatment. *P < 0.05, ***P < 0.0001, n = 10–13