Fig. 6

In a transient ischemic stroke rat model with delayed t-PA treatment, AGNHW treatment suppressed MMP-9 expression and activity, maintained microvascular integrity, and reserved the expression of collagen IV and tight junction protein claudin-5. A Representative gelatin zymography graph demonstrating MMP-9 activity in protein extracts from ischemic hemispheres. B Statistical analysis of MMP-9 activity of protein extracts from ischemic hemispheres. ***P < 0.001, ****P < 0.0001, n = 5 per group. C Representative immunostaining fluorescence of MMP-9, collagen IV, and tight junction claudin-5 in the ischemic hemispheres at about 6 mm away from the frontal tip