Fig. 5

Anemonin attenuates HT-29 cell inflammation via targeted regulation of PKC-θ. A RT-qPCR was used to detect whether PRKCQ overexpression plasmid upregulated PRKCQ level. B Western blot was used to determine whether PRKCQ overexpression plasmid upregulated PKC-θ protein expression. C mRNA levels of inflammatory factors were detected by RT-qPCR, following by transfection with pcPRKCQ or pcDNA3.1. D Western blot was used to detect the protein expression levels of inflammatory factors, following by transfection with pcPRKCQ or pcDNA3.1. E mRNA levels of inflammatory factors were detected by RT-qPCR, following by the transfected HT-29 cells stimulated with LPS with or without anemonin treatment. F Western blot was used to detect the protein expression levels of inflammatory factors, following by the transfected HT-29 cells stimulated with LPS with or without anemonin treatment. *p < 0.05, **p < 0.01, ***p < 0.001