Table 1 Primer sequences used for qRT-PCR
Forward primer | Reverse primer | |
|---|---|---|
Mouse IL-1β | 5’-TGCCACCTTTTGACAGTGATG-3’ | 5’-TTCTTGTGACCCTGAGCGAC-3’ |
Mouse TNF-α | 5’-ACCCTCACACTCACAAACCA-3’ | 5’-GAGGCAACCTGACCACTCTC-3’ |
Mouse IL-6 | 5’-GCCTTCTTGGGACTGATGCT-3’ | 5’-TGTGACTCCAGCTTATCTCTTGG-3’ |
Mouse GAPDH | 5’-GGGGTCCCAGCTTAGGTTCA-3’ | 5’-CCCAATACGGCCAAATCCGT-3’ |
Human IL-1β | 5’-AGAAGTACCTGAGCTCGCCA-3’ | 5’-TGGGATCTACACTCTCCAGC-3’ |
Human TNF-α | 5’-AGCCCATGTTGTAGCAAACC-3’ | 5’-ACATTGGGTCCCCCAGGATA-3’ |
Human IL-6 | 5’-TTCGGTCCAGTTGCCTTCTC-3’ | 5’-TCACCAGGCAAGTCTCCTCA-3’ |
Human PRKCQ | 5’-CACCGGGAAAAAGAGAGCCT-3’ | 5’-AGTGCTCTGTCGGCAAATGA-3’ |
Human PTPN2 | 5’-GAGGAGAACAGTGAGAGTGCT-3’ | 5’-TGGGCTTCAGGTCTTGCTTT-3’ |
Human GAPDH | 5’-CCATGTTGCAACCGGGAAG-3’ | 5’-GCCCAATACGACCAAATCAGAG-3’ |