Fig. 2

TSN did not inhibit the fusion process of autophagosome and lysosome. A Schematic diagram of RFP-GFP-LC3 reporter/LAMP1 reflected the fusion of autophagosome and lysosome. B Immunofluorescence photographs of the co-localization of LAMP1 (blue) and stably-expressing RFP-GFP-LC3 MDA-MB-231 cells treated with TSN (1 μM), Torin1 (100 nM), BAF (100 nM) or DMSO for 12 h. Nuclei were stained with Hoechst 33258. Scale bar: 10 μm