Fig. 3

GEN exerts its protective effect by activating AMPKα and suppressing NLRP3 inflammasome activity. A–D The mRNA expression of NLRP3, ASC, IL-1β, and IL-18 in mice myocardium of each group detected by RT-PCR (n = 5). E Representative western blotting results of NLRP3, ASC, N-GSDMD, cleaved caspase-1, and IL-1β (n = 6). F Densitometry data for NLRP3, ASC, N-GSDMD, cleaved caspase-1, and IL-1β in myocardium of each group of mice. G, H Inflammatory factor of IL-1β and IL-18 in the serum after GEN treatment (n = 5). I Myocardial caspase-1 activity was assessed in each group (n = 5). J Immunofluorescence TUNEL staining after GEN treatment (n = 6); scale bar = 20 μm. The data for each group were shown as the mean ± SD; ^#p < 0.05, ^##p < 0.01 vs. Sham group; *p < 0.05, **p < 0.01 vs. I/R + Vehicle group