Fig. 6

Geniposide inhibits the activation of the TXNIP/NLRP3 complex formation in NRVMs. A, B Translocation of TXNIP occurs in NRVMs. Immunofluorescence staining for TXNIP, where DAPI is used to locate the nuclei of the cells, and Western blotting of TXNIP in the 3 groups (n = 3). TXNIP located in nucleus in control group and shifted to cytoplasm in H/R + Vehicle group, and geniposide at 40 μM reversed the TXNIP localization from the cytoplasm to nucleus. C After reoxygenation, the interaction of TXNIP/NLRP3 was determined by coimmunoprecipitation (Co-IP) in the NRVMS of the 3 groups. Cardiomyocytes samples of the H/R + Vehicle and GEN 40 μM groups were harvested after reoxygenation (n = 3). D Quantitative analysis of TXNIP/NLRP3. The data for each group were shown as the mean ± SD; ^#p < 0.05, ^##p < 0.01 vs. Control group; *p < 0.05, **p < 0.01 vs. H/R + Vehicle group