Fig. 7

Effect of TXNIP and NLRP3 siRNA on inflammasome of H/R stimulated H9c2 cells. After application of negative control siRNA (NC siRNA) or TXNIP and NLRP3 siRNA for 48 h, H9c2 cells were exposed to hypoxia for another 3 h, and subsequently reoxygenation for 2 h. A–C Protein expression of TXNIP and NLRP3 was detected by Western blot at 48 h post-transfection, mRNA levels of TXNIP and NLRP3 in H9c2s were detected by RT-PCR at 24 h post-transfection and protein expression levels of ASC, N-GSDMD, cleaved Caspase-1 and IL-1β were analyzed by western blot. D, E Cell viability and LDH leakage after GEN treatment or using si-TXNIP or siNLRP3 (n = 3). F SOD2 enzymatic activity of H9c2 cells for the indicated groups, assayed using a commercial kit(n = 3). G IL-1β and IL-18 in cell supernatants were determined by ELISA assays. The data are presented as mean ± SD from three independent experiments. The data for each group were shown as the mean ± SD; ^#p < 0.05, ^##p < 0.01 vs. Control group; *p < 0.05, **p < 0.01 vs. H/R + Vehicle group