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Table 2 Primer sets for real-time RT-PCR

From: Inhibitory effect of Isatis tinctoria L. water extract on DNCB-induced atopic dermatitis in BALB/c mice and HaCaT cells

Name Forward Reverse
h-RANTES 5′ GATGCCAAAG AGAGAGGGAC 3′ 5′ AAATTTGTGT AAGTTCAGGT 3′
h-TARC 5′ CTGCACACAG AGACTCCCTC 3′ 5′ CTGGTACCAC GTCTTCAGCT 3′
h-MDC 5′ GAAACACTTC TACTGGACCT 3′ 5′ CAGGGAGGTA GGGCTCCTGA '
h-GAPDH 5′ TCAAGGCTGA GAACGGGAAG 3′ 5′ TGGACTCCAC GACGTACTCA 3′
m-TNF-α 5′ ATGAGCACAG AAAGCATGAT 3′ 5′ TACAGGCTTG TCACTCGAAT 3′
m-IL-6 5′ TTCCATCCAG TTGCCTTCTT 3′ 5′ ATTTCCACGA TTTCCCAGAG 3′
m-IL-13 5′ CATCTCCAAT TGCAATGCCA ′ 5′ GCCCAGGGAT GGTCTCTCCT 3′
m-GAPDH 5′ AACGACCCCT TCATTGAC 3′ 5′ TCCACGACAT ACTCAGCAC 3′
  1. h human, m mouse, RANTES regulated on activation normal T cell expressed and secreted, CCL5 TARC, thymus and activation-regulated chemokine, CCL17, MDC macrophage-derived chemokine, CCL22
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Chinese Medicine

ISSN: 1749-8546

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