Fig. 10

Effects of JTG on expression of associated proteins and NF-κB pathway of osteoclast induced from BMMs with RANKL and LPS. BMMs were incubated with RANKL and JTG for 48 h, the proteins were extracted to analyze associated proteins of osteoclast by Western blot. A: a Western blot imagines for expression of NFATc1, c-Fos, Cathepsin K and MMP9. A: b–e The quantification analysis of NFATc1, c-Fos, Cathepsin K and MMP9 based on the results of A: a by ECL detection system, respectively. B: a The images of Western blot for TRAF6, P-P65, P65 and IκBα. B: b–d The quantification analysis of TRAF6, P-P65/P65 and IκBα based on the results of B: a by using an ECL detection system, respectively. Each point represents the mean ± SD (n = 3). The experiments were repeated for three times. *P < 0.05, **P < 0.01 compared with control group