Fig. 7

Effects of JTG on osteoblast differentiation of BMSCs. A The BMSCs were treated with JTG for 48 h, the cell proliferation was assayed by CCK-8 method. B and C The BMSCs were treated with JTG for 7 d, the ALP activities and staining were performed by using assay kits. D The BMSCs were treated with JTG for 21 d, and stained with alizarin red to observe the bone nodules under microscope (×10), and E the bone nodules were dissolved with 10% (V/W) cetylpyridinium phosphate chloride, and the absorbance was measured at 570 nm with a microplate reader to quantify cell mineralization. The data were expressed as mean ± SD (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001 compared with control group