Fig. 9

Effects of JTG on formation and differentiation into osteoclasts of BMMs. The osteoclasts were induced from bone marrow macrophages (BMMs) of the femur in C57BL/6 mice with RANKL and M-CSF. A and B TRAP staining for osteoblast treated with JTG at concentration of 10, 20 and 40 μg/mL for 3 days. C Viability of osteoclasts treated with 10, 20 and 40 μg/mL JTG for 48 h was assessed by the MTT kits. D The number of TRAP-positive multinucleated osteoclast treated with JTG for 3 days. E F-actin rings of osteoclast treated with JTG for 48 h were stained with phalloidin and DAPI respectively, and then imaged with a fluorescence microscope (×630). F TRAP activities of osteoclast treated with JTG measured by p-nitrophenyl sodium phosphate method. The results are expressed as means ± SD (n = 6). The experiments were repeated for three times. *P < 0.05, **P < 0.01 compared with control group