Fig. 8
Effects of ASF on H2O2-induced apoptosis of PC12 cells. The percentage of apoptotic cells was measured by flow cytometry upon treatment with different concentrations of ASF for 24 h and subsequently exposure in H2O2 (175 μM)-treat for 4 h (A, B). Protein levels of Bcl-2 were analysed by Western blot (C). The gene expression levels of Bcl-2 (D), Caspase-3 (E), Bax (F) by RT-qPCR. Data were analysed as mean ± SE (n = 3). ##p < 0.01 and.###p < 0.001 versus control group; *p < 0.05, **p < 0.01 and ***p < 0.001 versus H2O2-stimulated group