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Fig. 7 | Chinese Medicine

Fig. 7

From: Comparison of ONT and CCS sequencing technologies on the polyploid genome of a medicinal plant showed that high error rate of ONT reads are not suitable for self-correction

Fig. 7

 A pattern of ONT reads self-correction. a For diploid, the homozygous base ‘A’ and the heterozygous base ‘C/T’ were corrected to be ‘A’ and ‘C’, respectively. Colorful blocks stand for conserved regions. b for tetraploid, in subgenome-A, reads r3 was sequenced “C->G” in error, and reads r11 of subgenome-B was “G->T”. As the existence of conserved regions (green and purple), reads r1–4, and r9–12 were clustered to perform error correction, resulting in two homozygous SNPs for the subgenomes using CCS reads and one heterozygous SNP using corrected ONT reads

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