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Table 1 Summary of VDL genome assemblies and mapping by NGS reads

From: Comparison of ONT and CCS sequencing technologies on the polyploid genome of a medicinal plant showed that high error rate of ONT reads are not suitable for self-correction

Assembly

CCS-hifiasm

ONT-nextdenovo

ONT-necat

ONT-wtdbg

Size (Gb)

3.55

4.28

3.75

3.45

N50 (Mb)

2.22

5.64

1.20

0.05

Complete BUSCOs (%)

92.63

90.09

87.92

77.51

NGS mapped rate (%)

99.60

99.60

99.52

99.29

NGS properly paired (%)

96.17

91.44

90.36

85.03

Depth (X)

36.62

30.25

34.36

35.52

Coverage (%)

99.53

75.70

76.31

49.15

Coverage % (≥ 5×)

98.26

69.92

71.12

45.60

Coverage % (≥ 10×)

93.50

61.99

64.11

40.01

Coverage % (≥ 20×)

72.31

42.18

45.90

33.32