Fig. 6

Compatibility of curcumin, piperine, and their combination with SH-SY5Y neuronal cells. (A) Experimental design. (B) Cell viability after treatment with EC₅₀, EC₇₅, and EC₉₀ concentrations of curcumin (4.0, 6.4, and 10.2 μM), piperine (20.0, 54.5, and 148.3 μM), and curcumin-piperine combination (1.2 + 5.8, 2.4 + 12.2, and 5.1 + 25.5 μM Cur and Pip). (C) Effect of curcumin, piperine, and their combination at EC₉₀ concentration on apoptosis and necrosis visualized by Hoechst 33342/PI double staining. Data are expressed as means ± S.E.M (n = 3). The differences between the control and treatment groups were analyzed using one-way ANOVA followed by Dunnett’s post hoc test. ***p < 0.001, **p < 0.01