Table 4 The stabilities of five analytes in rat serum under different storage conditions
Analytes | Concentration (ng/mL) | Sample stability (mean ± SD, %) | Three freeze–thaw stabilities (mean ± SD, %) | 4℃ short-term stabilities (mean ± SD, %) | − 80℃ long-term stabilities (mean ± SD, %) | room temperature short-term stabilities (mean ± SD, %) |
|---|---|---|---|---|---|---|
Timosaponin AIII | 1.48 | 91.95 ± 0.04 | 95.06 ± 0.02 | 100.7 ± 0.12 | 98.72 ± 0.19 | 96.95 ± 0.18 |
74.0 | 96.37 ± 1.13 | 101.8 ± 2.03 | 96.19 ± 0.81 | 96.55 ± 4.29 | 99.54 ± 2.19 | |
Glycyrrhizic acid | 14.9 | 99.11 ± 0.74 | 97.89 ± 1.79 | 91.11 ± 0.56 | 97.61 ± 1.98 | 112.8 ± 0.12 |
747 | 96.20 ± 5.96 | 102.0 ± 11.8 | 93.21 ± 9.08 | 100.38 ± 10.5 | 97.07 ± 3.97 | |
Liquiritin apioside | 2.88 | 100.9 ± 0.03 | 96.82 ± 0.08 | 99.05 ± 0.10 | 96.44 ± 0.01 | 100.4 ± 0.13 |
144 | 105.4 ± 3.08 | 101.3 ± 0.15 | 99.5 ± 1.74 | 98.97 ± 3.63 | 98.40 ± 1.38 | |
Mangiferin | 2.94 | 90.85 ± 0.06 | 96.28 ± 0.18 | 99.46 ± 0.34 | 102.9 ± 0.32 | 94.09 ± 0.18 |
147 | 106.2 ± 3.48 | 98.30 ± 2.40 | 97.90 ± 4.50 | 94.26 ± 8.31 | 98.02 ± 9.12 | |
Cinnamic acid | 14.8 | 110 ± 0.09 | 102.6 ± 1.21 | 109.7 ± 0.77 | 92.45 ± 0.79 | 98.33 ± 0.83 |
741 | 102.8 ± 5.93 | 102.4 ± 3.94 | 96.63 ± 14.4 | 97.52 ± 4.26 | 98.67 ± 3.50 |