Fig. 4

A Veen diagram of common gene targets of lung cancer and nitidine chloride. B Signaling pathways enriched terms in KEGG pathways. C Molecular docking analysis of NC targeting PI3K or Akt. D H1688 and A549 cells were treated with NC for 48 h, total cellular extracts were prepared and subjected to WB analysis using PI3K, p-PI3K, Akt, p-Akt and β-Actin antibodies. E CETSA was performed with cell lysates of H1688 or A549 cells that were treated with DMSO or NC with an increasing melting temperature (37 ℃-62 ℃, interval temperature: 5 ℃). F WB analysis of DARTS sample further revealed the increased stabilization of PI3K during proteolysis process in H1688 or A549 cells. Protein levels are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs control