Fig. 7

Hierarchical clustering heat map and OPLS-DA score map of differential metabolites. a Hierarchical clustering heat map of differential metabolites. The relative content in the figure is shown in different colors. The redder the color, the higher the expression level; the bluer the color, the lower the expression level. The columns represent samples, the rows represent metabolite names, and the cluster tree on the left of the figure is the differential metabolite cluster tree. b OPLS-DA score chart. The abscissa represents the first principal component interpretation degree, and the ordinate denotes the second principal component interpretation degree. Dots represent experimental samples, and colors symbolize different groups. The more concentrated the samples are within the group, the more scattered the samples are between the groups, indicating that the results are more reliable. c Comparison of differential metabolism of pyrotinib model group with the other three groups respectively. “ + ” represents the high content of differential metabolites in pyrotinib model group, and “−” represents the high content of differential metabolites in pyrotinib model group