Fig. 2

The distinct regulation on gut microbiome by TCM after 35 days intervention. Male C57BL/6J mice (5-week-old) were given Hot TCM (RG_LT, GJ_LT, FZ_LT) or Cold TCM (DH_LT, HQ_LT, HL_LT) by oral gavage once daily for 35 days. Mice in Control group were orally administrated with water (Con_LT). A Shannon index. B, C Principal coordinate analysis based on the Binary-Pearson distance algorithm on OTU level. D, E Fecal microbiota at phylum level by 16S rDNA sequencing under Mann–Whitney U test. F Heatmap shown the specific genus of Hot_LT and Cold_LT. G The number of regulated pathways in two TCM natures at KEGG pathway level 3. N = 5 each group, *P < 0.05, **P < 0.01