Fig. 2
bio-informatics results of high-througput RNA-seq analysis among H1688 cells with TCPT and their blank counterpart. A Volcano plot of DEGs between H1688 TCPT treatment group and its blank counterpart. The threshold of DEGs sets up as padj (corrected p value) < 0.05 and ∣log2foldchange∣ > 1. Down-regulated DEGs present as blue dots while up-regulated DEGs present as red dots. B Heatmap of of DEGs between H1688 blank vs H1688 TCPT treatment. Red block presents up-regulated DGEs, whereas blue block presents down-regulated DGEs. Upper and left dendrogram indicate the relationship of expression pattern and amount in individual samples, respectively. C Bar chart of total enriched GO terms of DEGs (rank top 30). The color of columns present category of biological process (BP), molecular function (MF) and cell component (CC), respectively. Ordinate shows the GO terms, and abscissa shows p-value of enrichment analysis. D Bubble diagram of KEGG enrichments of DEGs (Top 20). Ordinate shows the GO terms, and abscissa shows p-value of enrichment analysis. The size of bubble shows the enriched DEGs number, and the color of bubble presents the rich factor of each terms. Boxed terms are the representative of metabolism pathways (red box), signaling pathways(blue box) and types of cancer(orange box), respectively. E Classification of all terms to the category of metabolism pathways, signaling pathways or types of cancer. Ordinate shows the rich factor of each terms, abscissa and the size of bubble present proportion of subpsrietal DEGs of total DEGs, and color of size presents p-value of each terms. F Summary of novel genes and alternative splicing. Histogram of novel gene shows the length and number of found gene that cannot be referenced to library upper left panel). Venn chart indicates the types of alternative splicing (AS) among DEGs (lower left panel) and the event percentage of each type of total events(right panel). A3SS presents alternative 3’ splice site, A5SS presents alternative 5’ splice site, MEX presents mutually exclusive exons, RI presents retained intron and SE presents skipped exon. G KEGG Enrichment of AS. Ordinate shows the pathway terms, and abscissa shows p-value of enrichment analysis. The size of bubble shows the enriched DEGs number, and the color of bubble presents the rich factor of each terms. Red rectangle boxed the most DEGs enriched term-metabolic pathway