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Fig. 3 | Chinese Medicine

Fig. 3

From: Total coumarins of Pileostegia tomentella induces cell death in SCLC by reprogramming metabolic patterns, possibly through attenuating β-catenin/AMPK/SIRT1

Fig. 3

Master signaling pathway potentially regulates metabolism reprogram under TCPT treatment. A, B Validation of up- and down-regulated DEGs among H1688 blank, 50 μg/mL TCPT and 100 μg/mL TCPT using qPCR. DEGs in each chart are randomly selected from the DEGs list of RNA-seq. C Graphic KEGG map of two signaling axis. Details of annotation is labeled in the down part of graph, pink box illustrated the cellular workflow of β-catenin/AMPK/SIRT1 axis. D mRNA expression of 4 signaling proteins, EGFR, ERBB2, β-catenin and p53. E Protein expression and activation of β-catenin/AMPK/SIRT1 axis. F, G Rescue assay for detecting whether β-catenin might be the key target when treating TCPT in SCLC. Protein expressions and activation of β-catenin, AMPK and SIRT1 among H1688 with TCPT, combination of TCPT and si-β-catenin, and their counterparts, as well as H1688 with SKL2001 (agonist of β-catenin), combination of TCPT and SKL2001 and their counterparts. Tables in the right panel of F, G illustrate the relative reduction of expression of each proteins when compared with their no treatment counterparts. *, ** vs blank group, present P value < 0.05 and 0.01 respectively

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Chinese Medicine

ISSN: 1749-8546

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