Fig. 4

TCPT reprograms metabolic pattern in H1688. A Graphic KEGG mapping of 3 ATP-generated metabolism pathway, glycolysis, pentose phosphate pathway and aerobic oxidation. Details annotation are listed in left bottom. Metabolite labels in purple, orange and blue present as intermediate products from glycolysis, pentose phosphate pathway and aerobic oxidation, respectively. According to the results of KEGG mapping, enzymes with green dash line boxes are down-regulated DEGs, red dash line boxes are up-regulated DEGs and grey dash line boxes are non-DEGs. Arrows on green or red indicate the prediction of down- or up-trend of each metabolite. B The changes of protein expression of metabolic enzymes in H1688 cell and its TCPT treating counterparts. A series detection of metabolite or activity of key enzymes among H1688 blank, 50 μg/mL TCPT and 100 μg/mL TCPT groups are illustrated in intracellular glucose level (C), activity of HK (D), activity of LDH (E), intracellular and extracellular content of LA (F), activity of PDH (G) and intracellular content of ATP (H). *, ** vs blank group, present P value < 0.05 and 0.01 respectively